氢化可的松对奶牛乳腺上皮细胞酪蛋白合成的影响

doi:10.11843/j.issn.0366-6964.2014.10.012

Abstract

Abstract:

This study was conducted to investigate the effects of hydrocortisone (HYD) on growth，expressions of κ-casein (CSN3) and hydrocortisone receptor (NR3C1) genes and the relative quantities of κ-casein and total casein in bovine mammary epithelial cells.Bovine mammary epithelial cells from Chinese Holstein were used in an in vitro cultivation.Cells were cultured in a growth culture medium (without serum and hormone，control group)，and the growth culture medium supplemented with 0，1，10，100 and 1 000 ng•mL^-1 HYD，respectively.Changes of expression levels of CSN3 and NR3C1 genes and relative content of CSN3 and total casein were determined.The results showed as follows: 1) The cells proliferation in 1 000 ng•mL^-1 group was lower than these in the others in numerical value before 20 h，but significant difference was found in cell proliferation between groups after 20 h (P<0.05).2) The expression of CSN3 and NR3C1 gene were increased in 1-100 ng•mL^-1 HYD significantly (P<0.05).The relative quantities of κ-casein and total casein in 1 and 10 ng•mL^-1 groups were significantly higher than these in the other groups (P<0.05).3) A certain dose-response relationship existed between HYD and casein.That meant，small dose of HYD (1 ng•mL^-1) was more advantageous to promote the milk protein synthesis，but with the increase of HYD concentration，the promoting effects were gradually weakened and even reverse with the increase of HYD concentration (1 000 ng•mL^-1).The results indicated that with the increase of HYD concentration，the expression levels of CSN3 and NR3C1 genes and the relative quantities of CSN3 and total casein tended to be decreased，the optimal level was 1 ng•mL^-1.

CLC Number:

TIAN Qing，WANG Hong-rong，WANG Meng-zhi. Effects of HYD on the Synthesis of Casein in Mammary Epithelial Cells of Holstein Cows in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1663-1670.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.10.012

https://www.xmsyxb.com/EN/Y2014/V45/I10/1663

References

［1］孙晓静.奶牛泌乳相关激素周期性变化规律及激素对乳腺外植体中基因表达的影响［D］.泰安：山东农业大学，2012.
［2］WINTERMANTEL T M，BOCK D，FLEIG V，et al.The epithelial glucocorticoid receptor is required for the normal timing of cell proliferation during mammary lobuloalveolar development but is dispensable for milk production ［J］.Mol Endocrinol，2005,19(2):340-349. ［3］TUCKER H A.Symposium: hormnoal regulation of milk synthesis Hormones，mammary growth，and lactation :a 41-year perspective［J］.J Dairy Sci，2000,83(4):874-884.
［4］PLAUT K，BAUMAN D E，AGERGAARD N，et al.Effect of exogenous prolactin on lactational performance of dairy cows ［J］.Domest Anim Endocrinol，1987,4:279-290.
［5］CASEY T M，PLAUT K.The Role of glucocorticoids in secretory activation and milk secretion，a historical perspective［J］.J Mammary Gland Biol Neoplasia，2007,12:293-304.
［6］ZHOU Y,AKERS R M,JIANG H.Growth hormone can induce expression of four major milk protein genes in transfected MAC-T cells［J］.J Dairy Sci，2008,91:100-108.
［7］田青,王洪荣.胰岛素、催乳素和氢化可的松对奶牛乳腺上皮细胞增殖和凋亡的影响［J］.中国饲料，2013,2:8-12.
［8］赵珂.奶牛乳腺上皮细胞葡萄糖摄取的调控及其对乳成分合成的影响研究［D］.杭州：浙江大学，2011.
［9］王皓宇,秦彤,郝海生,等.胰岛素对体外培养奶牛乳腺上皮细胞乳蛋白、乳脂肪合成相关基因mRNA表达的影响［J］.畜牧兽医学报,2013,44（5）:710-718.
［10］ERDMANN B，BRETER H.Irregular distribution of mammary derived growth inhibitor in the bovine mammary epithelium ［J］.Cell Tissue Res,1993,272(2):383-389．
［11］ACCORNERO P，MARTIGNANI E，MIRETTI S，et al.Epidermal growth factor and hepatocyte growth factor receptors collaborate to induce multiple biological responses in bovine mammary epithelial cells ［J］.J Dairy Sci，2009,92(8)：3667-3675．
［12］佟慧丽,高学军,李庆章,等.胰岛素、催乳素对奶山羊乳腺上皮细胞泌乳功能的影响［J］.畜牧兽医学报,2008,39（6）:721-725．
［13］李敏.新型糖皮质激素对肥大细胞脱颗粒的快速作用及其机制研究［D］.上海：第二军医大学，2010．
［14］HOUDEBINE L M，DJIANE J，DUSANTER-FOURT I,et al.Hormonal action controllingmammary activity［J］.J Dairy Sci，1985,68:489-500.
［15］SHEEHY P A,NICHOLAS K R,WYMM P C.Aninvestigation of the role of insulin in bovine milk protein gene expression in mammary explants culture［J］.Asian-Aus J Anim Sci，2000,Supplement(13):272-275．
［16］SHAO Y,WALL E H,MCFADDEN T B,et al.Lactogenic hormones stimulate expression of lipogenicgenes but not glucose transporters in bovine mammary gland［J］.Domest Anim Endocrinol,2013,44(2):57-69.
［17］文静,卜登攀,王建发,等.激素调控乳蛋白合成的作用及其分子机制［J］.华北农学报,2012，27(增刊)：111-115.
［18］SHAH O J,KIMBALL S R,JEFFERSON L S.Glucocorticoids abate p70(S6k) and eIF4E function in L6 skeletal myoblasts［J］.Am J Physiol-Endocrinol Metabol,2000,279:E74-E82.
［19］徐柏林，王梦芝,张兴夫,等.精氨酸水平对奶牛乳腺上皮细胞体外生长及κ-酪蛋白基因表达的影响［J］.动物营养学报，2012，24(5)：852-858.
［20］季昀,庞学燕,田青,等.生长激素和胰岛素样生长因子Ⅰ对奶牛乳蛋白合成关键激酶及调节因子mRNA表达量的影响［J］.动物营养学报，2013，25(1)：198-207.
［21］季昀.GH和IGF-I对奶牛乳腺上皮细胞中酪蛋白合成的调节作用机制［D］.扬州：扬州大学，2013.
［22］李忠秋.牛乳腺上皮细胞研究进展［J］．黑龙江农业科学,2008,5:130-133.
［23］刘畅，赵锋，李庆章.不同泌乳相关激素和生长因子对奶牛乳腺上皮细胞增殖的影响［J］.中国畜牧兽医，2012,39（5）：102-106.
［24］王春燕.瘦素对奶牛乳腺上皮细胞乳蛋白合成的调控作用研究［D］.呼和浩特：内蒙古农业大学，2012.
［25］王秀美.二、三维培养模式对奶牛乳腺上皮细胞泌乳代谢相关基因的影响［D］.呼和浩特：内蒙古农业大学，2013.
［26］周苗苗.奶牛乳腺中小肽的摄取及其在乳蛋白合成中的作用［D］.杭州：浙江大学，2011.

[1]	ZHAO Shanjiang, SHUAI Zhiqiang, PANG Yunwei, HAO Haisheng, ZOU Huiying, DU Weihua, ZHAO Xueming, ZHU Huabin. Effect of Age at First Pregnancy on Milk Production and Reproductive Performance During the First 2 Lactations in Holstein Dairy Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 732-742.
[2]	ZHAO Chang, ZHANG Jiang, BAI Yunlong, SUN Shuhan, SONG Yuxi, XIA Cheng. Analysis of Serum Differential Proteins in Cows with Inactive Ovaries Based on iTRAQ Technology [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 972-982.
[3]	LI Xiao-jun, LI Xin, CHEN Xiao-li, ZHAO Yi-qiang, LU Yong-qiang, WANG Dong. The Transcription of Pld6 during Spermiogenesis and the Analysis of Its Protein Sequence in Dairy Cows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2632-2640.
[4]	GUO Yan-sheng, TAO Jin-zhong. Selection of Milk Biomarkers of Pregnancy Recognition in Dairy Cows Based on LC-Q/TOF-MS Metabolomics [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1633-1641.
[5]	LI Lan-qi, LIU Jiang-jing, CHEN Xiao-li, LI Xin, LU Yong-qiang, WANG Shui-lian, WANG Dong. Study on the Activity Alteration of Dairy Cow during Estrus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1387-1393.
[6]	LIU Ying-chun, CHEN Yu-meng, HAN Zhe-xian, GAO Feng, ZHOU Huan-min. Induction and Identification of Neural Cell from Mammary Stem Cells in Holstein Cows and the Effect of Prolactin on Its Proliferative Activity [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 542-548.
[7]	XU Chao, LI Feng, HAO Hai-sheng, LIU Yan, ZHAO Xue-ming, PANG Yun-wei, ZHU Hua-bin, DU Wei-hua. Sex-Related Genes Expression during Development of the Early Bovine Fetuses [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1892-1901.
[8]	ZHAO Yan-kun, SHAO Wei, WANG Li-wen, YU Xiong. Effect of Co-culturing with Umbilical Cord Mesenchymal Stem Cells on Milk Fat Synthesis and Expression of Key Genes in Bovine Mammary Gland Epithelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 846-853.
[9]	LIU Xiao-qian，LIU Ying, RONG Chao，WANG Kun，XIAO Hang，ZHANG Yuan-shu. Expression Localization of Angiotensin Converting Enzyme 2 (ACE 2) in Dairy Cow Mammary Gland and Its Anti-inflammation Effect [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(3): 552-560.
[10]	LI Xiao-jun，WANG Zhen-ling，CHEN Xiao-li，KOU Hong-xiang，LI Xiu-ling，WANG Yan，WANG Dong. Study Progress on the Rule of Body Temperature and Its Application in Reproduction of Dairy Cattle [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2331-2341.
[11]	LI Yan,XUE Qian，GAO Yan-xia，CHEN Zi-ning，LI Qiu-feng，LI Jian-guo. Effects of Rumen-protected Glucose on Performance and Blood Biochemical Parameters during Postpartum Period in Dairy Cows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 113-119.
[12]	CAO Lu，WANG Ze-zhao，DONG Gang-hui，WANG Ya-jing，GUO Gang，WANG Ya-chun. Knowledge Mapping Analysis on Thermal Stress Research of Dairy Cattle [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1489-1495.
[13]	LIU Hong，YANG Hong-bo，ZHU Jian-ming，LIANG Xiang-huan，ZHAN Jin-shun，LIN Miao，ZHAO Guo-qi. Effects of Cysteamine Hydrochloride on Apparent Nutrient Digestibility，Serum Biochemical and Antioxidant Indices of Lactating Dairy Cows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(3): 416-423.
[14]	CHENG Lei，WANG Ding-fa，LIU Xiao-hua，XIANG Min，LING Ming-hu，HU Xiu-zhong，XIA Yu. Study on ISG15 and OAS1 Transcriptions in Peripheral Blood of Dairy Cows during Early Pregnancy [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 77-84.
[15]	LI Qiu-feng，LI Jian-guo，WU Yun-hai，MA Ya-bin，LIU Xing-hua，GAO Yan-xia，CAO Yu-feng，LIU Ting-yu. The Effects of Vitamin E，Beta-carotene and Zinc on Semen Quality，Antioxidant Indicators in Hot Weather [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 587-595.

Effects of HYD on the Synthesis of Casein in Mammary Epithelial Cells of Holstein Cows in vitro

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments